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M94B0804.TXT
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1994-11-11
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Document 0804
DOCN M94B0804
TI Requirement of the human immunodeficiency virus type 1 env gene sequence
for TAR-independent trans activation by Tat from the major
immediate-early promoter of murine cytomegalovirus.
DT 9412
AU Kim YS; McArdle Laboratory for Cancer Research, University of
Wisconsin,; Madison, 53706.
SO Biochem Biophys Res Commun. 1994 Sep 15;203(2):1152-9. Unique Identifier
: AIDSLINE MED/94380023
AB Previously it has been demonstrated that the human immunodeficiency
virus type 1 (HIV-1) Tat protein mediates induction of the HIV-1 env
expression through a TAR-independent manner in heterologous and
homologous promoter systems (Kim and Risser, 1993, J. Virol. 67, 239;
Kim and Panganiban, 1993, J. Virol. 67, 3739). To further explore the
transactivation of HIV-1 env gene, I examined expression of the env, the
bacterial CAT, and the firefly luciferase genes from a heterologous
promoter, the major immediate-early promoter (MIEP) of murine
cytomegalovirus (MCMV). Here we show that Tat augments gene expression
from the MCMV MIEP only when linked to the env gene. Surprisingly, in
contrast to the expression from an HIV-1 LTR lacking the TAR element,
TAR-independent transactivation of env gene expression from MCMV MIEP
did not require the full length Tat protein. In addition, deletion of
the previously identified cis-acting Tat-responsive element in env did
not affect Tat transactivation of the env gene expression. Thus, there
are multiple distinct elements that mediate Tat responsiveness in the
absence TAR.
DE Bacterial Proteins/PHARMACOLOGY Base Sequence
Cytomegalovirus/*GENETICS Gene Expression Gene Products,
tat/*PHARMACOLOGY *Genes, env Hela Cells Human HIV-1/*GENETICS
Immediate-Early Proteins/*GENETICS Membrane Proteins/PHARMACOLOGY
Molecular Sequence Data *Promoter Regions (Genetics) Repetitive
Sequences, Nucleic Acid Support, U.S. Gov't, P.H.S. *Trans-Activation
(Genetics) JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).